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A

addParameter(String) - Method in class qupath.ext.biop.cellpose.CellposeBuilder
Generic means of adding a cellpose parameter
addParameter(String, String) - Method in class qupath.ext.biop.cellpose.CellposeBuilder
Generic means of adding a cellpose parameter

B

batchSize(Integer) - Method in class qupath.ext.biop.cellpose.CellposeBuilder
Defines the batch size for training
build() - Method in class qupath.ext.biop.cellpose.CellposeBuilder
Create a Cellpose2D, all ready for detection.
build() - Method in class qupath.ext.biop.cellpose.OpCreators.ImageNormalizationBuilder
Build a OpCreators.TileOpCreator according to the builder's parameters.
builder(File) - Static method in class qupath.ext.biop.cellpose.Cellpose2D
Load a previously serialized builder.
builder(String) - Static method in class qupath.ext.biop.cellpose.Cellpose2D
Create a builder to customize detection parameters.

C

cellConstrainScale - Variable in class qupath.ext.biop.cellpose.Cellpose2D
 
cellConstrainScale(double) - Method in class qupath.ext.biop.cellpose.CellposeBuilder
Constrain any cell expansion defined using CellposeBuilder.cellExpansion(double) based upon the nucleus size.
cellExpansion - Variable in class qupath.ext.biop.cellpose.Cellpose2D
 
cellExpansion(double) - Method in class qupath.ext.biop.cellpose.CellposeBuilder
Amount by which to expand detected nuclei to approximate the cell area.
Cellpose2D - Class in qupath.ext.biop.cellpose
Dense object detection based on the cellpose and omnipose publications
Cellpose2D() - Constructor for class qupath.ext.biop.cellpose.Cellpose2D
 
CellposeBuilder - Class in qupath.ext.biop.cellpose
Cell detection based on the following method:
CellposeBuilder(File) - Constructor for class qupath.ext.biop.cellpose.CellposeBuilder
can create a cellpose builder from a serialized JSON version of this builder.
CellposeBuilder(String) - Constructor for class qupath.ext.biop.cellpose.CellposeBuilder
Build a cellpose model by providing a string which can be the name of a pretrained model or a path to a custom model
cellposeChannels(Integer, Integer) - Method in class qupath.ext.biop.cellpose.CellposeBuilder
Explicitly set the cellpose channels manually.
CellposeExtension - Class in qupath.ext.biop.cellpose
Install Cellpose as an extension.
CellposeExtension() - Constructor for class qupath.ext.biop.cellpose.CellposeExtension
 
cellposeSetup - Variable in class qupath.ext.biop.cellpose.Cellpose2D
 
CellposeSetup - Class in qupath.ext.biop.cellpose
 
CellposeSetup() - Constructor for class qupath.ext.biop.cellpose.CellposeSetup
 
cellprobThreshold(Double) - Method in class qupath.ext.biop.cellpose.CellposeBuilder
/** cellprob threshold, default is 0, decrease to find more and larger masks
channels(int...) - Method in class qupath.ext.biop.cellpose.CellposeBuilder
Specify channels.
channels(String...) - Method in class qupath.ext.biop.cellpose.CellposeBuilder
Specify channels by name.
channels(ColorTransforms.ColorTransform...) - Method in class qupath.ext.biop.cellpose.CellposeBuilder
Define the channels (or color transformers) to apply to the input image.
classify(String) - Method in class qupath.ext.biop.cellpose.CellposeBuilder
Request that a classification is applied to all created objects.
classify(PathClass) - Method in class qupath.ext.biop.cellpose.CellposeBuilder
Request that a classification is applied to all created objects.
compartments - Variable in class qupath.ext.biop.cellpose.Cellpose2D
 
compartments(ObjectMeasurements.Compartments...) - Method in class qupath.ext.biop.cellpose.CellposeBuilder
Specify the compartments within which intensity measurements are made.
compute(Mat) - Method in class qupath.ext.biop.cellpose.OpCreators.PercentileTileOpCreator
 
compute(Mat) - Method in class qupath.ext.biop.cellpose.OpCreators.ZeroMeanVarianceTileOpCreator
 
CONDA - qupath.ext.biop.cmd.VirtualEnvironmentRunner.EnvType
 
constrainToParent - Variable in class qupath.ext.biop.cellpose.Cellpose2D
 
constrainToParent(boolean) - Method in class qupath.ext.biop.cellpose.CellposeBuilder
If true, constrain nuclei and cells to any parent annotation (default is true).
createAnnotations() - Method in class qupath.ext.biop.cellpose.CellposeBuilder
Create annotations rather than detections (the default).
createOps(ImageDataOp, ImageData<BufferedImage>, ROI, ImagePlane) - Method in interface qupath.ext.biop.cellpose.OpCreators.TileOpCreator
Compute the (tile-based) ops from the image.
creatorFun - Variable in class qupath.ext.biop.cellpose.Cellpose2D
 

D

detectObjects(ImageData<BufferedImage>, Collection<? extends PathObject>) - Method in class qupath.ext.biop.cellpose.Cellpose2D
Detect cells within one or more parent objects, firing update events upon completion.
detectObjectsImpl(ImageData<BufferedImage>, Collection<? extends PathObject>) - Method in class qupath.ext.biop.cellpose.Cellpose2D
Detect cells within one or more parent objects, firing update events upon completion.
diameter(Double) - Method in class qupath.ext.biop.cellpose.CellposeBuilder
The estimated diameter of the objects to detect.
downsample(double) - Method in class qupath.ext.biop.cellpose.OpCreators.ImageNormalizationBuilder
Specify the downsample factor to use when calculating the normalization.

E

epochs(Integer) - Method in class qupath.ext.biop.cellpose.CellposeBuilder
Defines the number of epochs for training
eps(double) - Method in class qupath.ext.biop.cellpose.OpCreators.ImageNormalizationBuilder
Error constant used for numerical stability and avoid dividing by zero.
excludeEdges() - Method in class qupath.ext.biop.cellpose.CellposeBuilder
Exclude on edges.
EXE - qupath.ext.biop.cmd.VirtualEnvironmentRunner.EnvType
 

F

flowThreshold(Double) - Method in class qupath.ext.biop.cellpose.CellposeBuilder
Flow error threshold, 0 turns off this optional QC step.

G

getCellposePytonPath() - Method in class qupath.ext.biop.cellpose.CellposeSetup
 
getDescription() - Method in class qupath.ext.biop.cellpose.CellposeExtension
 
getDescription() - Method in enum qupath.ext.biop.cmd.VirtualEnvironmentRunner.EnvType
 
getInstance() - Static method in class qupath.ext.biop.cellpose.CellposeSetup
 
getName() - Method in class qupath.ext.biop.cellpose.CellposeExtension
 
getOmniposePytonPath() - Method in class qupath.ext.biop.cellpose.CellposeSetup
 
getOutputLog() - Method in class qupath.ext.biop.cellpose.Cellpose2D
Returns the log from running the cellpose command, with any error messages and status updates of the cellpose process You can use this during training or prediction
getQCResults() - Method in class qupath.ext.biop.cellpose.Cellpose2D
Get the results table associated with the Quality Control run
getQuPathVersion() - Method in class qupath.ext.biop.cellpose.CellposeExtension
 
getRepository() - Method in class qupath.ext.biop.cellpose.CellposeExtension
 
getTrainingDirectory() - Method in class qupath.ext.biop.cellpose.Cellpose2D
The directory that was used for saving the training images
getTrainingResults() - Method in class qupath.ext.biop.cellpose.Cellpose2D
Returns a parsed version of the cellpose log as a ResultsTable with columns Epoch, Time, Loss, Loss Test and LR
getValidationDirectory() - Method in class qupath.ext.biop.cellpose.Cellpose2D
The directory that was used for saving the validation images
globalPathClass - Variable in class qupath.ext.biop.cellpose.Cellpose2D
 
globalPreprocess - Variable in class qupath.ext.biop.cellpose.Cellpose2D
 
groundTruthDirectory(File) - Method in class qupath.ext.biop.cellpose.CellposeBuilder
Specify the training directory

I

ignoreCellOverlaps - Variable in class qupath.ext.biop.cellpose.Cellpose2D
 
ignoreCellOverlaps(boolean) - Method in class qupath.ext.biop.cellpose.CellposeBuilder
If true, ignore overlaps when computing cell expansion.
imageNormalizationBuilder() - Static method in class qupath.ext.biop.cellpose.Cellpose2D
Build a normalization op that can be based upon the entire (2D) image, rather than only local tiles.
imageNormalizationBuilder() - Static method in class qupath.ext.biop.cellpose.OpCreators
Build a normalization op that can be based upon the entire (2D) image, rather than only local tiles.
ImageNormalizationBuilder() - Constructor for class qupath.ext.biop.cellpose.OpCreators.ImageNormalizationBuilder
 
inputAdd(double...) - Method in class qupath.ext.biop.cellpose.CellposeBuilder
Add an offset as a preprocessing step.
inputScale(double...) - Method in class qupath.ext.biop.cellpose.CellposeBuilder
Multiply by a scale factor as a preprocessing step.
inputSubtract(double...) - Method in class qupath.ext.biop.cellpose.CellposeBuilder
Subtract an offset as a preprocessing step.
installExtension(QuPathGUI) - Method in class qupath.ext.biop.cellpose.CellposeExtension
 

L

learningRate(Double) - Method in class qupath.ext.biop.cellpose.CellposeBuilder
Defines the learning rate

M

maskThreshold(Double) - Method in class qupath.ext.biop.cellpose.CellposeBuilder
maxDimension(int) - Method in class qupath.ext.biop.cellpose.OpCreators.ImageNormalizationBuilder
The maximum width or height, which is used to calculate a downsample factor for the image if OpCreators.ImageNormalizationBuilder.downsample(double) is not specified.
measureIntensity() - Method in class qupath.ext.biop.cellpose.CellposeBuilder
Request default intensity measurements are made for all available cell compartments.
measureIntensity(Collection<ObjectMeasurements.Measurements>) - Method in class qupath.ext.biop.cellpose.CellposeBuilder
Request specified intensity measurements are made for all available cell compartments.
measurements - Variable in class qupath.ext.biop.cellpose.Cellpose2D
 
measureShape - Variable in class qupath.ext.biop.cellpose.Cellpose2D
 
measureShape() - Method in class qupath.ext.biop.cellpose.CellposeBuilder
Request shape measurements are made for the detected cell or nucleus.
minTrainMasks(Integer) - Method in class qupath.ext.biop.cellpose.CellposeBuilder
Excludes training data with less than n training masks (n labels)
model - Variable in class qupath.ext.biop.cellpose.Cellpose2D
 
modelDirectory - Variable in class qupath.ext.biop.cellpose.Cellpose2D
 
modelDirectory(File) - Method in class qupath.ext.biop.cellpose.CellposeBuilder
Define the directory where the newly trained model should be saved

N

noCellposeNormalization() - Method in class qupath.ext.biop.cellpose.CellposeBuilder
convenience method? to deactivate cellpose normalization.
normalizePercentiles(double, double) - Method in class qupath.ext.biop.cellpose.CellposeBuilder
Apply percentile normalization separately to the input image channels.
normalizePercentiles(double, double, boolean, double) - Method in class qupath.ext.biop.cellpose.CellposeBuilder
Apply percentile normalization to the input image channels, or across all channels jointly.
normalizePercentilesGlobal(double, double, double) - Method in class qupath.ext.biop.cellpose.CellposeBuilder
Convenience method to call global normalization for the dataset
nThreads - Variable in class qupath.ext.biop.cellpose.Cellpose2D
 
nThreads(int) - Method in class qupath.ext.biop.cellpose.CellposeBuilder
Specify the number of threads to use for processing.

O

op - Variable in class qupath.ext.biop.cellpose.Cellpose2D
 
OpCreators - Class in qupath.ext.biop.cellpose
Helper class for creating new ImageOps based upon other image properties.
OpCreators() - Constructor for class qupath.ext.biop.cellpose.OpCreators
 
OpCreators.ImageNormalizationBuilder - Class in qupath.ext.biop.cellpose
Builder for a OpCreators.TileOpCreator that can be used for image preprocessing using min/max percentiles or zero-mean-unit-variance normalization.
OpCreators.PercentileTileOpCreator - Class in qupath.ext.biop.cellpose
Tile op creator that computes offset and scale values across the full image to normalize using min and max percentiles.
OpCreators.TileOpCreator - Interface in qupath.ext.biop.cellpose
Helper class for creating (tile-based) ImageOps with parameters that are derived from an entire image or ROI.
OpCreators.ZeroMeanVarianceTileOpCreator - Class in qupath.ext.biop.cellpose
Tile op creator that computes offset and scale values across the full image to normalize to zero mean and unit variance.
OTHER - qupath.ext.biop.cmd.VirtualEnvironmentRunner.EnvType
 
overlap - Variable in class qupath.ext.biop.cellpose.Cellpose2D
 

P

parameters - Variable in class qupath.ext.biop.cellpose.Cellpose2D
 
percentiles(double, double) - Method in class qupath.ext.biop.cellpose.OpCreators.ImageNormalizationBuilder
Specify min and max percentiles to calculate normalization values.
perChannel(boolean) - Method in class qupath.ext.biop.cellpose.OpCreators.ImageNormalizationBuilder
Compute the normalization values separately per channel; if false, values are computed jointly across channels.
pixelSize - Variable in class qupath.ext.biop.cellpose.Cellpose2D
 
pixelSize(double) - Method in class qupath.ext.biop.cellpose.CellposeBuilder
Resolution at which the cell detection should be run.
preprocess - Variable in class qupath.ext.biop.cellpose.Cellpose2D
 
preprocess(OpCreators.TileOpCreator) - Method in class qupath.ext.biop.cellpose.CellposeBuilder
Add an OpCreators.TileOpCreator to generate preprocessing operations based upon the entire image, rather than per tile.
preprocess(ImageOp...) - Method in class qupath.ext.biop.cellpose.CellposeBuilder
Add preprocessing operations, if required.

Q

qupath.ext.biop.cellpose - package qupath.ext.biop.cellpose
 
qupath.ext.biop.cmd - package qupath.ext.biop.cmd
 

R

runCommand() - Method in class qupath.ext.biop.cmd.VirtualEnvironmentRunner
This builds, runs the command and outputs it to the logger as it is being run

S

saveBuilder(String) - Method in class qupath.ext.biop.cellpose.CellposeBuilder
Save this builder as a JSON file in order to be able to reuse it in place
saveTrainingImages() - Method in class qupath.ext.biop.cellpose.Cellpose2D
Save training images for the project
setArguments(List<String>) - Method in class qupath.ext.biop.cmd.VirtualEnvironmentRunner
This is the code you actually want to run after 'python'.
setCellposePytonPath(String) - Method in class qupath.ext.biop.cellpose.CellposeSetup
 
setOmniposePytonPath(String) - Method in class qupath.ext.biop.cellpose.CellposeSetup
 
setOverlap(int) - Method in class qupath.ext.biop.cellpose.CellposeBuilder
Set the overlap (in pixels) between tiles.
showTrainingGraph() - Method in class qupath.ext.biop.cellpose.Cellpose2D
Overloaded method for backwards compatibility.
showTrainingGraph(boolean, boolean) - Method in class qupath.ext.biop.cellpose.Cellpose2D
Displays a JavaFX graph as a dialog, so you can inspect the Losses per epoch also saves the graph to the QC folder if requested
simplify(double) - Method in class qupath.ext.biop.cellpose.CellposeBuilder
Customize the extent to which contours are simplified.
simplifyDistance - Variable in class qupath.ext.biop.cellpose.Cellpose2D
 

T

tempDirectory(File) - Method in class qupath.ext.biop.cellpose.CellposeBuilder
Specify the temporary directory
tileHeight - Variable in class qupath.ext.biop.cellpose.Cellpose2D
 
tileSize(int) - Method in class qupath.ext.biop.cellpose.CellposeBuilder
Size in pixels of a tile used for detection.
tileSize(int, int) - Method in class qupath.ext.biop.cellpose.CellposeBuilder
Size in pixels of a tile used for detection.
tileWidth - Variable in class qupath.ext.biop.cellpose.Cellpose2D
 
toString() - Method in enum qupath.ext.biop.cmd.VirtualEnvironmentRunner.EnvType
 
train() - Method in class qupath.ext.biop.cellpose.Cellpose2D
Executes the cellpose training by 1.
trainDirectory - Variable in class qupath.ext.biop.cellpose.Cellpose2D
 

U

useMask(boolean) - Method in class qupath.ext.biop.cellpose.OpCreators.ImageNormalizationBuilder
Optionally use any ROI mask provided for the calculation.
useOmnipose() - Method in class qupath.ext.biop.cellpose.CellposeBuilder
Use Omnipose implementation: Adds --omni flag to command

V

valDirectory - Variable in class qupath.ext.biop.cellpose.Cellpose2D
 
valueOf(String) - Static method in enum qupath.ext.biop.cmd.VirtualEnvironmentRunner.EnvType
Returns the enum constant of this type with the specified name.
values() - Static method in enum qupath.ext.biop.cmd.VirtualEnvironmentRunner.EnvType
Returns an array containing the constants of this enum type, in the order they are declared.
VENV - qupath.ext.biop.cmd.VirtualEnvironmentRunner.EnvType
 
VirtualEnvironmentRunner - Class in qupath.ext.biop.cmd
A wrapper to run python virtualenvs, that tries to figure out the commands to run based on the environment type
VirtualEnvironmentRunner(String, VirtualEnvironmentRunner.EnvType, String) - Constructor for class qupath.ext.biop.cmd.VirtualEnvironmentRunner
 
VirtualEnvironmentRunner.EnvType - Enum in qupath.ext.biop.cmd
This enum helps us figure out the type of virtualenv.

Z

zeroMeanUnitVariance() - Method in class qupath.ext.biop.cellpose.OpCreators.ImageNormalizationBuilder
Normalize for zero mean and unit variance.
zeroMeanUnitVariance(boolean) - Method in class qupath.ext.biop.cellpose.OpCreators.ImageNormalizationBuilder
Optionally normalize for zero mean and unit variance.
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